Recombinant:Article Title: Functional diversity of dopamine axons in prefrontal cortex during classical conditioning
Article Snippet: .. 91136 13 of 23 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- GFP (Rabbit Polyclonal) Thermo Fisher Scientific Cat# A- 11122, RRID:AB_221569 IF (1:1000) Antibody Anti- Tyrosine Hydroxylase (Sheep Polyclonal) Abcam Cat# ab113, RRID:AB_297905 IF (1:200) Antibody Anti- Rabbit IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (Donkey Polyclonal) Thermo Fisher Scientific Cat# A- 21206, RRID:AB_2535792 IF (1:500) Antibody Anti- Sheep IgG (H+L) Antibody, Alexa Fluor 568 Conjugated (Donkey Polyclonal) Thermo Fisher Scientific Cat# A- 21099, RRID:AB_2535753 IF (1:500) Recombinant DNA reagent hSynapsin1- FLEx- axon- jGCaMP8m (plasmid) This paper Addgene #216533 Described at ‘Headplate implant and virus injection’ of ‘Surgery’ section Strain, strain background (AAV) AAV2/1- hSynapsin1- FLEx- axon- jGCaMP8m- WPRESV40 University of South Carolina Viral Vectors Core Genetic reagent (mouse) Mouse: Slc6a3tm1.1(cre)Bkmn The Jackson Laboratory JAX: 006660 Chemical compound Normal Donkey Serum Sigma- Aldrich Cat# D9663, RRID:AB_2810235 Software, algorithm MATLAB Mathworks RRID: SCR_001622 https://www. mathworks.com Software, algorithm Python Mathworks RRID:SCR_008394 https://www.anaconda. com/ Software, algorithm Suite2p Patriarchi et al., 2018; MouseLand, 2024 https://github.com/ MouseLand/suite2p Software, algorithm DeepLabCut Mathis et al., 2018 https://github. com/DeepLabCut/ DeepLabCut; Mathis et al., 2024 Software, algorithm B- spline Grid, Image and Point based Registration Dirk- Jan Kroon https://jp.mathworks. com/matlabcentral/ fileexchange/20057b-spline-grid-imageand-point-basedregistration Software, algorithm Pytorch, TorchVision Meta AI https://pytorch.org/ Other Mouse Brain Connectivity Atlas Allen Brain Map https://connectivity. brain-map.org/ Further details are provided in the caption of Figure 1—figure supplement 1. ..
Plasmid Preparation:Article Title: Functional diversity of dopamine axons in prefrontal cortex during classical conditioning
Article Snippet: .. 91136 13 of 23 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- GFP (Rabbit Polyclonal) Thermo Fisher Scientific Cat# A- 11122, RRID:AB_221569 IF (1:1000) Antibody Anti- Tyrosine Hydroxylase (Sheep Polyclonal) Abcam Cat# ab113, RRID:AB_297905 IF (1:200) Antibody Anti- Rabbit IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (Donkey Polyclonal) Thermo Fisher Scientific Cat# A- 21206, RRID:AB_2535792 IF (1:500) Antibody Anti- Sheep IgG (H+L) Antibody, Alexa Fluor 568 Conjugated (Donkey Polyclonal) Thermo Fisher Scientific Cat# A- 21099, RRID:AB_2535753 IF (1:500) Recombinant DNA reagent hSynapsin1- FLEx- axon- jGCaMP8m (plasmid) This paper Addgene #216533 Described at ‘Headplate implant and virus injection’ of ‘Surgery’ section Strain, strain background (AAV) AAV2/1- hSynapsin1- FLEx- axon- jGCaMP8m- WPRESV40 University of South Carolina Viral Vectors Core Genetic reagent (mouse) Mouse: Slc6a3tm1.1(cre)Bkmn The Jackson Laboratory JAX: 006660 Chemical compound Normal Donkey Serum Sigma- Aldrich Cat# D9663, RRID:AB_2810235 Software, algorithm MATLAB Mathworks RRID: SCR_001622 https://www. mathworks.com Software, algorithm Python Mathworks RRID:SCR_008394 https://www.anaconda. com/ Software, algorithm Suite2p Patriarchi et al., 2018; MouseLand, 2024 https://github.com/ MouseLand/suite2p Software, algorithm DeepLabCut Mathis et al., 2018 https://github. com/DeepLabCut/ DeepLabCut; Mathis et al., 2024 Software, algorithm B- spline Grid, Image and Point based Registration Dirk- Jan Kroon https://jp.mathworks. com/matlabcentral/ fileexchange/20057b-spline-grid-imageand-point-basedregistration Software, algorithm Pytorch, TorchVision Meta AI https://pytorch.org/ Other Mouse Brain Connectivity Atlas Allen Brain Map https://connectivity. brain-map.org/ Further details are provided in the caption of Figure 1—figure supplement 1. ..
Virus:Article Title: Functional diversity of dopamine axons in prefrontal cortex during classical conditioning
Article Snippet: .. 91136 13 of 23 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- GFP (Rabbit Polyclonal) Thermo Fisher Scientific Cat# A- 11122, RRID:AB_221569 IF (1:1000) Antibody Anti- Tyrosine Hydroxylase (Sheep Polyclonal) Abcam Cat# ab113, RRID:AB_297905 IF (1:200) Antibody Anti- Rabbit IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (Donkey Polyclonal) Thermo Fisher Scientific Cat# A- 21206, RRID:AB_2535792 IF (1:500) Antibody Anti- Sheep IgG (H+L) Antibody, Alexa Fluor 568 Conjugated (Donkey Polyclonal) Thermo Fisher Scientific Cat# A- 21099, RRID:AB_2535753 IF (1:500) Recombinant DNA reagent hSynapsin1- FLEx- axon- jGCaMP8m (plasmid) This paper Addgene #216533 Described at ‘Headplate implant and virus injection’ of ‘Surgery’ section Strain, strain background (AAV) AAV2/1- hSynapsin1- FLEx- axon- jGCaMP8m- WPRESV40 University of South Carolina Viral Vectors Core Genetic reagent (mouse) Mouse: Slc6a3tm1.1(cre)Bkmn The Jackson Laboratory JAX: 006660 Chemical compound Normal Donkey Serum Sigma- Aldrich Cat# D9663, RRID:AB_2810235 Software, algorithm MATLAB Mathworks RRID: SCR_001622 https://www. mathworks.com Software, algorithm Python Mathworks RRID:SCR_008394 https://www.anaconda. com/ Software, algorithm Suite2p Patriarchi et al., 2018; MouseLand, 2024 https://github.com/ MouseLand/suite2p Software, algorithm DeepLabCut Mathis et al., 2018 https://github. com/DeepLabCut/ DeepLabCut; Mathis et al., 2024 Software, algorithm B- spline Grid, Image and Point based Registration Dirk- Jan Kroon https://jp.mathworks. com/matlabcentral/ fileexchange/20057b-spline-grid-imageand-point-basedregistration Software, algorithm Pytorch, TorchVision Meta AI https://pytorch.org/ Other Mouse Brain Connectivity Atlas Allen Brain Map https://connectivity. brain-map.org/ Further details are provided in the caption of Figure 1—figure supplement 1. ..
Injection:Article Title: Functional diversity of dopamine axons in prefrontal cortex during classical conditioning
Article Snippet: .. 91136 13 of 23 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- GFP (Rabbit Polyclonal) Thermo Fisher Scientific Cat# A- 11122, RRID:AB_221569 IF (1:1000) Antibody Anti- Tyrosine Hydroxylase (Sheep Polyclonal) Abcam Cat# ab113, RRID:AB_297905 IF (1:200) Antibody Anti- Rabbit IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (Donkey Polyclonal) Thermo Fisher Scientific Cat# A- 21206, RRID:AB_2535792 IF (1:500) Antibody Anti- Sheep IgG (H+L) Antibody, Alexa Fluor 568 Conjugated (Donkey Polyclonal) Thermo Fisher Scientific Cat# A- 21099, RRID:AB_2535753 IF (1:500) Recombinant DNA reagent hSynapsin1- FLEx- axon- jGCaMP8m (plasmid) This paper Addgene #216533 Described at ‘Headplate implant and virus injection’ of ‘Surgery’ section Strain, strain background (AAV) AAV2/1- hSynapsin1- FLEx- axon- jGCaMP8m- WPRESV40 University of South Carolina Viral Vectors Core Genetic reagent (mouse) Mouse: Slc6a3tm1.1(cre)Bkmn The Jackson Laboratory JAX: 006660 Chemical compound Normal Donkey Serum Sigma- Aldrich Cat# D9663, RRID:AB_2810235 Software, algorithm MATLAB Mathworks RRID: SCR_001622 https://www. mathworks.com Software, algorithm Python Mathworks RRID:SCR_008394 https://www.anaconda. com/ Software, algorithm Suite2p Patriarchi et al., 2018; MouseLand, 2024 https://github.com/ MouseLand/suite2p Software, algorithm DeepLabCut Mathis et al., 2018 https://github. com/DeepLabCut/ DeepLabCut; Mathis et al., 2024 Software, algorithm B- spline Grid, Image and Point based Registration Dirk- Jan Kroon https://jp.mathworks. com/matlabcentral/ fileexchange/20057b-spline-grid-imageand-point-basedregistration Software, algorithm Pytorch, TorchVision Meta AI https://pytorch.org/ Other Mouse Brain Connectivity Atlas Allen Brain Map https://connectivity. brain-map.org/ Further details are provided in the caption of Figure 1—figure supplement 1. ..
Bioprocessing:Article Title: Functional diversity of dopamine axons in prefrontal cortex during classical conditioning
Article Snippet: .. 91136 13 of 23 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- GFP (Rabbit Polyclonal) Thermo Fisher Scientific Cat# A- 11122, RRID:AB_221569 IF (1:1000) Antibody Anti- Tyrosine Hydroxylase (Sheep Polyclonal) Abcam Cat# ab113, RRID:AB_297905 IF (1:200) Antibody Anti- Rabbit IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (Donkey Polyclonal) Thermo Fisher Scientific Cat# A- 21206, RRID:AB_2535792 IF (1:500) Antibody Anti- Sheep IgG (H+L) Antibody, Alexa Fluor 568 Conjugated (Donkey Polyclonal) Thermo Fisher Scientific Cat# A- 21099, RRID:AB_2535753 IF (1:500) Recombinant DNA reagent hSynapsin1- FLEx- axon- jGCaMP8m (plasmid) This paper Addgene #216533 Described at ‘Headplate implant and virus injection’ of ‘Surgery’ section Strain, strain background (AAV) AAV2/1- hSynapsin1- FLEx- axon- jGCaMP8m- WPRESV40 University of South Carolina Viral Vectors Core Genetic reagent (mouse) Mouse: Slc6a3tm1.1(cre)Bkmn The Jackson Laboratory JAX: 006660 Chemical compound Normal Donkey Serum Sigma- Aldrich Cat# D9663, RRID:AB_2810235 Software, algorithm MATLAB Mathworks RRID: SCR_001622 https://www. mathworks.com Software, algorithm Python Mathworks RRID:SCR_008394 https://www.anaconda. com/ Software, algorithm Suite2p Patriarchi et al., 2018; MouseLand, 2024 https://github.com/ MouseLand/suite2p Software, algorithm DeepLabCut Mathis et al., 2018 https://github. com/DeepLabCut/ DeepLabCut; Mathis et al., 2024 Software, algorithm B- spline Grid, Image and Point based Registration Dirk- Jan Kroon https://jp.mathworks. com/matlabcentral/ fileexchange/20057b-spline-grid-imageand-point-basedregistration Software, algorithm Pytorch, TorchVision Meta AI https://pytorch.org/ Other Mouse Brain Connectivity Atlas Allen Brain Map https://connectivity. brain-map.org/ Further details are provided in the caption of Figure 1—figure supplement 1. ..
Software:Article Title: Functional diversity of dopamine axons in prefrontal cortex during classical conditioning
Article Snippet: .. 91136 13 of 23 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- GFP (Rabbit Polyclonal) Thermo Fisher Scientific Cat# A- 11122, RRID:AB_221569 IF (1:1000) Antibody Anti- Tyrosine Hydroxylase (Sheep Polyclonal) Abcam Cat# ab113, RRID:AB_297905 IF (1:200) Antibody Anti- Rabbit IgG (H+L) Antibody, Alexa Fluor 488 Conjugated (Donkey Polyclonal) Thermo Fisher Scientific Cat# A- 21206, RRID:AB_2535792 IF (1:500) Antibody Anti- Sheep IgG (H+L) Antibody, Alexa Fluor 568 Conjugated (Donkey Polyclonal) Thermo Fisher Scientific Cat# A- 21099, RRID:AB_2535753 IF (1:500) Recombinant DNA reagent hSynapsin1- FLEx- axon- jGCaMP8m (plasmid) This paper Addgene #216533 Described at ‘Headplate implant and virus injection’ of ‘Surgery’ section Strain, strain background (AAV) AAV2/1- hSynapsin1- FLEx- axon- jGCaMP8m- WPRESV40 University of South Carolina Viral Vectors Core Genetic reagent (mouse) Mouse: Slc6a3tm1.1(cre)Bkmn The Jackson Laboratory JAX: 006660 Chemical compound Normal Donkey Serum Sigma- Aldrich Cat# D9663, RRID:AB_2810235 Software, algorithm MATLAB Mathworks RRID: SCR_001622 https://www. mathworks.com Software, algorithm Python Mathworks RRID:SCR_008394 https://www.anaconda. com/ Software, algorithm Suite2p Patriarchi et al., 2018; MouseLand, 2024 https://github.com/ MouseLand/suite2p Software, algorithm DeepLabCut Mathis et al., 2018 https://github. com/DeepLabCut/ DeepLabCut; Mathis et al., 2024 Software, algorithm B- spline Grid, Image and Point based Registration Dirk- Jan Kroon https://jp.mathworks. com/matlabcentral/ fileexchange/20057b-spline-grid-imageand-point-basedregistration Software, algorithm Pytorch, TorchVision Meta AI https://pytorch.org/ Other Mouse Brain Connectivity Atlas Allen Brain Map https://connectivity. brain-map.org/ Further details are provided in the caption of Figure 1—figure supplement 1. ..
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